Buffers for restriction enzymes

Restriction enzymes perform their function under the suitable conditions. In particular, the temperature and the compositions of the conditions are important. Vendors supply various buffers, some buffers are identical, but the others are different.

Similar buffers


Buffer chart

Low-salt buffers

TakaraL10mM Tris-HCl7.5none10mM MgCl21mM DTT-
Nippon geneL10mM Tris-HCl7.5none10mM MgCl21mM DTT-
NEB110mM Bis-Tris-HCl7.0none10mM MgCl21mM DTT-
FermentasB10mM Tris-HCl7.5none10mM MgCl2-0.1mg/mL BSA
InvitrogenREact150mM Tris-HCl8.0none10mM MgCl2--
PromegaA6mM Tris-HCl7.56mM NaCl6mM MgCl21mM DTT-

Mid-salt buffers

TakaraM10mM Tris-HCl7.550mM NaCl10mM MgCl21mM DTT-
Nippon geneM10mM Tris-HCl7.550mM NaCl10mM MgCl21mM DTT-
NEB210mM Tris-HCl7.950mM NaCl10mM MgCl21mM DTT-
FermentasG10mM Tris-HCl7.550mM NaCl10mM MgCl2-0.1mg/mL BSA
InvitrogenREact250mM Tris-HCl8.050mM NaCl10mM MgCl2--
PromegaC10mM Tris-HCl7.950mM NaCl10mM MgCl21mM DTT-

High-salt buffers

TakaraH50mM Tris-HCl7.5100mM NaCl10mM MgCl21mM DTT-
Nippon geneH50mM Tris-HCl7.5100mM NaCl10mM MgCl21mM DTT-
NEB350mM Tris-HCl7.9100mM NaCl10mM MgCl21mM DTT-
FermentasO50mM Tris-HCl7.5100mM NaCl10mM MgCl2-0.1mg/mL BSA
InvitrogenREact350mM Tris-HCl8.0100mM NaCl10mM MgCl2--
PromegaE6mM Tris-HCl7.5100mM NaCl6mM MgCl21mM DTT-

KCl buffers

TakaraK20mM Tris-HCl8.5100mM KCl10mM MgCl21mM DTT-
FermentasR10mM Tris-HCl8.5100mM KCl10mM MgCl2-0.1mg/mL BSA
Invitrogen650mM Tris-HCl7.450mM KCl/50mM NaCl6mM MgCl2--
PromegaJ10mM Tris-HCl7.550mM KCl7mM MgCl21mM DTT-

Acetic acid buffers

TakaraT33mM Tris-OAc7.966mM KOAc10mM MgOAc20.5mM DTT-
Nippon geneA20mM Tris-OAc7.950mM KOAc210mM MgOAc1mM DTT-
NEB420mM Tris-OAc7.950mM KOAc10mM MgOAc21mM DTT-
FermentasY33mM Tris-OAc7.966mM KOAc10mM MgOAc2-0.1mg/mL

Buffer list of vendors


NamePropertybufferpHsaltmetal ionreducer
LLow salt10mM Tris-HCl7.5none10mM MgCl21mM DTT
MMid salt10mM Tris-HCl7.550mM NaCl10mM MgCl21mM DTT
HHigh salt50mM Tris-HCl7.5100mM NaCl10mM MgCl21mM DTT
KKCl salt20mM Tris-HCl8.5100mM KCl10mM MgCl21mM DTT
TTrisOAC33mM Tris-OAc7.966mM KOAc10mM MgOAc20.5mM DTT

Nippon gene

NamePropertybufferpHsaltmetal ionreducer
LLow salt10mM Tris-HCl7.5none10mM MgCl21mM DTT
MMid salt10mM Tris-HCl7.550mM NaCl10mM MgCl21mM DTT
HHigh salt50mM Tris-HCl7.5100mM NaCl10mM MgCl21mM DTT
AAceticacid20mM Tris-OAc7.950mM KOAc10mM MgOAc21mM DTT
BBasic pH10mM Tris-HCl8.5100mM NaCl10mM MgCl21mM DTT


NamePropertybufferpHsaltmetal ionreducer
1low salt10mM Bis-Tris-HCl7.0none10mM MgCl21mM DTT
2mid salt10mM Tris-HCl7.950mM NaCl10mM MgCl21mM DTT
3high salt50mM Tris-HCl7.9100mM NaCl10mM MgCl21mM DTT
4aceticacid20mM Tris-OAc7.950mM KOAc10mM MgOAc21mM DTT


NamePropertybufferpHsaltmetal ionreducerother
BBlue10mM Tris-HCl7.5none10mM MgCl2-0.1mg/mL BSA
GGreen10mM Tris-HCl7.550mM NaCl10mM MgCl2-0.1mg/mL BSA
OOrange50mM Tris-HCl7.5100mM NaCl10mM MgCl2-0.1mg/mL BSA
RRed10mM Tris-HCl8.5100mM KCl10mM MgCl2-0.1mg/mL BSA
YYellow33mM Tris-OAc7.966mM KOAc10mM MgOAc2-0.1mg/mL


NamePropertybufferpHsaltmetal ionreducerother
1Low salt50mM Tris-HCl8.0-10mM MgCl2--
2Mid salt50mM Tris-HCl8.050mM NaCl10mM MgCl2--
3High salt50mM Tris-HCl8.0100mM NaCl10mM MgCl2--
4KCl20mM Tris-HCl7.450mM KCl5mM MgCl2--
6KCl/NaCl50mM Tris-HCl7.450mM KCl/50mM NaCl6mM MgCl2--
7KCl/NaCl50mM Tris-HCl8.050mM KCl/50mM NaCl10mM MgCl2--
8Low salt20mM Tris-HCl7.4-10mM MgCl2--
9High salt100mM Tris-HCl7.6150mM NaCl10mM MgCl2--

(*)10mM DTT seperately supplied.


NamePropertybuffer at 37ºCpHsaltmetal ionreducerother
ALow salt6mM Tris-HCl7.56mM NaCl6mM MgCl21mM DTT-
BMid salt6mM Tris-HCl7.550mM NaCl6mM MgCl21mM DTT-
CMid salt10mM Tris-HCl7.950mM NaCl10mM MgCl21mM DTT-
DHigh salt6mM Tris-HCl7.9150mM NaCl6mM MgCl21mM DTT-
EHigh salt6mM Tris-HCl7.5100mM NaCl6mM MgCl21mM DTT-
FHigh salt10mM Tris-HCl8.5100mM NaCl10mM MgCl21mM DTT-
GBase pH50mM Tris-HCl8.2-5mM MgCl2--
HStrong buffering90mM Tris-HCl7.550mM NaCl10mM MgCl2--
JMid KCl10mM Tris-HCl7.550mM KCl7mM MgCl21mM DTT-
KHigh KCl10mM Tris-HCl7.4150mM KCl10mM MgCl2--
LBase/High salt10mM Tris-HCl9.0100mM NaCl3mM MgCl2--



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