BioWiki

DpnI

DpnI cuts sites 'GATC' if the adenine is methylated.

Recognition GATC
thermTemp. 37 ºC
microtubeBufferLowMidHighAcKCl
≥75% X X X O X

Reaction

DpnI cuts sites 'GmATC' (mA: N6-methyladenine). The structures below are part of dam-methylated pUC19 and non-methylated pUC19, respectively.

Methylated - reactiveUnmethylated - inert
Methylated Unmethylated

Comparison with DpnII and MboI

DpnII and MboI are isoscizomers that recognize a same sequence and cut at same position. These REases can cut unmethylated sequence.

Methylated - inertUnmethylated - reactive
Methylated Unmethylated

Links