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HEPES

HEPES is one of the Goods' buffers.

Name HEPES1)
Molecular Formula C8H18N2O4S
Molecular Weight 238.306
CAS No. 7365-45-9
pKa(25ºC) 7.48

PubChem image 23831

Preparation

1M HEPES-KOH

  1. Disolve HEPES(238.3 g) in 800 ml H2O.
  2. Adjust pH by addition of KOH.
  3. Add H2O upto 1 L.
  4. If neccessary, autoclave.
  5. Store at 4°C.
1) 2-[4-(2-Hydroxyethyl)-1-piperazinyl] ethanesulfonic acid