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Sau3AI

Recognition GATC
thermTemp. 37 ºC
microtubeBufferLowMidHighAcKCl
≥75% O O O O X

Isoscizomer

The recognition sites of isoscizomer, MboI will be blocked by dam methylase. On the other hand, Sau3A can cut the GATC sites even if the adenines are methylated. Therefore, Sau3AI is used for mapping or construction of library of methylated genome.

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