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TAE buffer

TAE buffer is Tris-acetate buffer containing EDTA. It is used for agarose gel electrophoreses.

Preparation

50x TAE

Mix the following solutes and adjust to 1L by H2O.
Store this stock solution at room temperature and dillute on your using.
0.5x TAE works well for agarose gel electrophoresis.

Composition
Tris242 g
Acetic acid57.1 mL
EDTA・2Na (2H2O)18.6 g
Final concentration
50x TAE2M Tris-acetate/ 50mM EDTA
working (1x)40 mM Tris-acetate / 1 mM EDTA

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