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TBE buffer

TBE buffer is Tris-borate buffer containing EDTA for gel electrophoreses.

Preparation

10x TBE

Mix the followings and adjust the volume to 1L. Store at room temperature and dillute on your using.

Tris108 g
Boric acid55 g
EDTA・2Na (2H2O)3.7 g
Concentration
10x TBE890mM Tris-borate/ 20mM EDTA
Working (1x)89 mM Tris-borate / 2 mM EDTA

0.5x TBE works well for agarose GE.

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