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TE buffer

This solution is used for stock of DNA. EDTA is strong chelator of metal ions that are cofactors of endonucleases.

Preparation

  1. Mix two stock solution, 1M Tris-HCl and 0.5M EDTA.
  2. Add H2O upto 1L.
stock solution
1M Tris-HCl (pH8.0)10 mL
0.5 M EDTA (pH8.0)2 mL

⇒ final concentration: 10 mM Tris-HCl, 1 mM EDTA pH 8.0