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REases for 5'-sticky ends

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AccI AccI Recognition [GTMKAC] Temp. 37 ºC BufferLowMidHighAcKCl ≥75% X X X O X * GTATAC / GTCGAC * M,K Links * Restriction enzyme * HincII * SalI * Buffer for restriction enzymes * REBASE restriction_enzyme 5_sticky ,
AvaI AvaI Recognition [CYCGRG] Temp. 37 ºC BufferLowMidHighAcKCl ≥75% X X X O X * GTCGAG / CCCGGG * R,Y Links * Restriction enzyme * SmaI * Buffer for restriction enzymes * REBASE restriction_enzyme 5_sticky ,
AvaII AvaII Recognition [GGWCC] Temp. 37 ºC BufferLowMidHighAcKCl ≥75% X O X O X * GGACC / GGTCC * W Links * Restriction enzyme * Buffer for restriction enzymes * REBASE restriction_enzyme 5_sticky ,
BamHI BamHI Recognition [GGATCC] Temp. 37 ºC BufferLowMidHighAcKCl ≥75% X O X X X Links * Restriction enzyme * M.BamHI * Buffer for restriction enzymes * REBASE restriction_enzyme 5_sticky ,
BstEII BstEII Recognition [GTNACC] Temp. 60 ºC BufferLowMidHighAcKCl ≥75% X X X O X * Nucleotide N * Origin:Bacillus stearothermophilus ET Links * Restriction enzyme * Buffer for restriction enzymes * REBASE restriction_enzyme 5_sticky not_37_c_reaction , ,
EcoRI EcoRI Recognition [GAATTC] Temp. 37 ºC BufferLowMidHighAcKCl ≥75% O O O O X Links * Restriction enzyme * M.EcoRI * Buffer for restriction enzymes * REBASE restriction enzyme 5_sticky , ,
HindIII HindIII Recognition [AAGCTT] Temp. 37 °C BufferLowMidHighAcKCl ≥75% X O X X X Links * Restriction enzyme * Buffer for restriction enzymes * REBASE restriction_enzyme 5_sticky ,
MboI MboI Recognition [GATC] Temp. 37 ºC BufferLowMidHighAcKCl ≥75% X O O O O Isoscizomer Sau3AI Sau3AI is an isoscizomer of MboI. Sau3AI can cut methylated sites, so it is often used for partial digestion of genome DNA. Links * Restriction enzyme * Sau3AI * DpnI * ,
NarI NarI Recognition [GGCGCC] Temp 37 °C BufferLowMidHighAcKCl ≥75% O X X X X Links * Restriction enzyme * Buffer for restriction enzymes * REBASE restriction_enzyme 5_sticky ,
NdeI NdeI Recognition [CATATG] Temp. 37 °C BufferLowMidHighAcKCl ≥75% X X O O X Links * Restriction enzyme * Buffer for restriction enzymes * REBASE restriction_enzyme 5_sticky ,
SalI SalI Recognition [GTCGAC] Temp. 37 °C BufferLowMidHighAcKCl ≥75% reactive X X O X X Links * Restriction enzyme * HincII * AccI * TaqI * Buffer for restriction enzymes * REBASE restriction_enzyme 5_sticky ,
Sau3AI Sau3AI Recognition [GATC] Temp. 37 ºC BufferLowMidHighAcKCl ≥75% O O O O X Isoscizomer The recognition sites of isoscizomer, MboI will be blocked by dam methylase. On the other hand, Sau3A can cut the GATC sites even if the adenines are methylated. Therefore, Sau3AI is used for mapping or construction of library of methylated genome. ,
TaqI TaqI Recognition [TCGA] Temperature 65 ºC BufferLowMidHighAcKCl ≥75% X X O O X * Origin: Thermus aquaticus YTI Links * Buffer for restriction enzymes * REBASE restriction_enzyme 5_sticky not_37_c_reaction , ,
XbaI XbaI Recognition [TCTAGA] Temp. 37 °C BufferLowMidHighAcKCl ≥75% X O X X X Links * Restriction enzyme * Buffer for restriction enzymes * REBASE restriction_enzyme 5_sticky ,