ADA (buffer) ADA (buffer) tmp Adenosine deaminase Name Adenosine deaminase EC3.5.4.4KEGG PathwayPurine metabolism Links * ADA - Japanese buffer
Boric acid Boric acid Links * TBE buffer acid buffer ,
Buffer solutions Buffer solutions Good's buffer Good et al. reported that the Zwitterionic buffers are suitable for biological experiments. They have the following characters. * They have physiological range of pKa(6.0 - 9.0). * The pKa values of them are not influenced by temperature and concentration of them. ,
CHES CHES CHES is one of a Good's buffer. Name CHES Molecular Formula CAS No.103-47-9pKa(20ºC)9.55 buffer
Comparison between TAE and TBE Comparison between TAE and TBE There are some differences between TAE buffer and TBE buffer. Generally, 1xTAE or 0.5xTAE is used for agarose gel electrophoresis, whereas 1xTBE is used for PAGE. Comparison Recovery of nucleic acids TAE has been prefavorably used so far because of the poor recovery of nucleic acids from TBE-gel. However, recent commercial recovery kits utilizing silica works well for either TAE-gel or TBE-gel.
Edetic acid Edetic acid Preparation 0.5M EDTA (pH 8.0) EDTA-2Na-2 186.1 g700 mL (final 1L) * Solve EDTA by adding NaOH pelette, and adjust pH to 8.0. * Add to 1 L. * Autoclave. If 1L of EDTA stock solution is too much, scale down according to your experiments. ,
HEPES HEPES HEPES is one of the Goods' buffers. Name HEPES Molecular Formula Molecular Weight 238.306CAS No. 7365-45-9 pKa(25ºC) 7.48 Preparation 1M HEPES-KOH * Disolve HEPES(238.3 g) in 800 ml . * Adjust pH by addition of KOH. ,
MES MES buffer
MOPS MOPS Name MOPS Molecular Weight 209.2633 g/mol Molecular Formula XLogP -1.2 pKa(25ºC) 7.17 buffer
Phosphate buffer Phosphate buffer Phosphate buffer is often used for column chlomatography. But it is not an ideal buffer for biological assays because phosphate can act as either a substrate or a regulation factor of enzymes. Besides, the buffering ability decreases significantly at low pH. ,
Phosphate buffered saline Phosphate buffered saline * Disolve the following chemicals in approx. 800 mL of . * NaCl 80 g * KCl 2 g * * Wikipedia * Phosphate buffered saline Phosphate buffered saline (abbreviated PBS) is a buffer solution commonly used in biochemistry. It is a salty solution containing sodium chloride, sodium phosphate and potassium phosphate. The buffer helps to maintain a constant pH. The concentration usually matches the human body (isotonic).
PIPES PIPES One of the Good's buffers with pKa of 7.48 (25ºC)。 buffer
TAE buffer TAE buffer TAE buffer is Tris-acetate buffer containing EDTA. It is used for agarose gel electrophoreses. Preparation 50x TAE Mix the following solutes and adjust to 1L by . Store this stock solution at room temperature and dillute on your using. ,
TBE buffer TBE buffer TBE buffer is Tris-borate buffer containing EDTA for gel electrophoreses. Preparation 10x TBE Mix the followings and adjust the volume to 1L. Store at room temperature and dillute on your using. Tris108 gBoric acid55 gEDTA・2Na (2)3.7 gConcentration ,
TE buffer TE buffer This solution is used for stock of DNA. EDTA is strong chelator of metal ions that are cofactors of endonucleases. Preparation * Mix two stock solution, 1M Tris-HCl and 0.5M EDTA. * Add upto 1L. stock solution 1M Tris-HCl (pH8.0)10 mL0.5 M EDTA (pH8.0)2 mL => final concentration: 10 mM Tris-HCl, 1 mM EDTA pH 8.0 ,