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T4 DNA polymerase

T4 DNA polymerase has two activities, 5'→3' polymerase activity and 3'→5' single-strand specific exonuclease activity. T4 DNA polymerase is often used for to do blunting sticky ends.

Reaction

3'→5' exonuclease
activity
5'→3'polymerase
activity
nuclease activitypolymerase activity

The activities of T4 DNA polymerase are utilized to blunt sticky ends.

  1. 5' sticky end → filled-in by the polymerase activity.
  2. 3' sticky end → digested by exonuclease activity.

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