Blunt ends
| ページ | 内容 | タグ |
|---|---|---|
| AluI | AluI Recognition [AGCT] Temp. 37 ºC BufferLowMidHighAcKCl ≥75% X O X O X Links * Restriction enzyme * Buffer for restriction enzymes * REBASE restriction_enzyme blunt | restriction enzyme, blunt |
| Blunting sticky ends | Blunting sticky ends Sticky-ends can be blunted with appropriate enzymes Following enzymatic activities are utilized for blunting of DNA ends. * 5'->3' polymerase activity to 'fill-in'. * Deletion by exonuclease activity. * Digestion by endonuclease. | blunt, polymerase, nuclease |
| DpnI | DpnI DpnI cuts sites 'GATC' if the adenine is methylated. Recognition [GATC] Temp. 37 ºC BufferLowMidHighAcKCl ≥75% X X X O X Reaction DpnI cuts sites 'GmATC' (mA: N6-methyladenine). The structures below are part of dam-methylated pUC19 and non-methylated pUC19, respectively. | restriction enzyme, blunt |
| DraI | DraI Recognition [TTTAAA] Temp. 37 ºC BufferLowMidHighAcKCl ≥75% X O X O X Links * Restriction enzyme * Buffer for restriction enzymes * REBASE restriction_enzyme blunt | restriction enzyme, blunt |
| HincII | HincII Recognition [GTCGAC] Temp. 37 °C BufferLowMidHighAcKCl ≥75% reactive X O O O X Links * Restriction enzyme * SalI * AccI * Buffer for restriction enzymes * REBASE restriction_enzyme blunt | restriction enzyme, blunt |
| MspA1I | MspA1I Recognition [CMGCKG] Temp. 37 °C BufferLowMidHighAcKCl ≥75% X X X O X * CAGCTG / CCGCGG * Nucleotide M,K Links * Restriction enzyme * Buffer for restriction enzymes * REBASE restriction_enzyme blunt | restriction enzyme, blunt |
| PvuII | PvuII Recognition [CAGCTG] Temp. 37 °C BufferLowMidHighAcKCl ≥75% O O O X O Links * Restriction enzyme * AluI * MspA1I * Buffer for restriction enzymes * REBASE restriction_enzyme blunt | restriction enzyme, blunt |
| RsaI | RsaI Recognition [GTAC] Temp. 37 °C BufferLowMidHighAcKCl ≥75% O O X O X Links * Restriction enzyme * Buffer for restriction enzymes * REBASE restriction_enzyme blunt | restriction enzyme, blunt |
| ScaI | ScaI Recognition [AGTACT] Temp. 37 °C BufferLowMidHighAcKCl ≥75% o o O X X Links * Restriction enzyme * Buffer for restriction enzymes * REBASE restriction_enzyme blunt | restriction enzyme, blunt |
| SmaI | SmaI Recognition [CCCGGG] Temp. 25 ºC BufferLowMidHighAcKCl ≥75% X O O O X Links * Restriction enzyme * AvaI * Buffer for restriction enzymes * REBASE restriction_enzyme blunt not_37_c_reaction | restriction enzyme, blunt, not 37 c reaction |
| SspI | SspI Recognition [AATATT] Temp. 37 °C BufferLowMidHighAcKCl ≥75% X o X X X Links * Restriction enzyme * Buffer for restriction enzymes * REBASE restriction_enzyme blunt | restriction enzyme, blunt |
| StuI | StuI Recognition [AGGCCT] Temp. 37 °C BufferLowMidHighAcKCl ≥75% o O X o X Links * Buffer for restriction enzymes * REBASE restriction_enzyme blunt | restriction enzyme, blunt |
最終更新: 2013/01/31 14:38
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