Ampicillin Ampicillin Ampicillin is an antibiotics that inhibits synthesis of cell-wall. Resistance * β-lactamase * InterPro entry Preparation * Solve sodium ampicillin 5g in or 20%EtOH aq. * Adjust the volume to 50mL. * Sterilize using filter and dispense into 1mL vials. * Store at -20°C. * On using, add 1mL (1vial) into 1L medium. ,
Buffer solutions Buffer solutions Good's buffer Good et al. reported that the Zwitterionic buffers are suitable for biological experiments. They have the following characters. * They have physiological range of pKa(6.0 - 9.0). * The pKa values of them are not influenced by temperature and concentration of them. ,
Chloramphenicol Chloramphenicol Chloramphenicol is an antibiotic that inhibits translation of protein from mRNA. It binds A site in a ribosome 50S subunit and inhibits peptidyltransferase activity. Resistance * Chloramphenicol acetyltransferase * IPR001707 Preparation * Solve chloramphenicol 1.5g in methanol and adjust to 50mL. ,
Edetic acid Edetic acid Preparation 0.5M EDTA (pH 8.0) EDTA-2Na-2 186.1 g700 mL (final 1L) * Solve EDTA by adding NaOH pelette, and adjust pH to 8.0. * Add to 1 L. * Autoclave. If 1L of EDTA stock solution is too much, scale down according to your experiments. ,
Ethidium bromide Ethidium bromide A trypanocidal agent and possible antiviral agent that is widely used in experimental cell biology and biochemistry. Ethidium has several experimentally useful properties including binding to nucleic acids, noncompetitive inhibition of nicotinic acetylcholine receptors, and fluorescence among others. It is most commonly used as the bromide. ,
G418 (Geneticin) G418 (Geneticin) G418 is an inhibitor of protein synthesis. Molecular Weight 496.553 g/mol Molecular Formula Resistance * Neor/Kanr (Aminoglycoside phosphotransferase) * InterPro entry Preparation G418 is available either solid or aqueous solution (50mg/mL) The activity of solid G418 decreases to 70% activity ,
HEPES HEPES HEPES is one of the Goods' buffers. Name HEPES Molecular Formula Molecular Weight 238.306CAS No. 7365-45-9 pKa(25ºC) 7.48 Preparation 1M HEPES-KOH * Disolve HEPES(238.3 g) in 800 ml . * Adjust pH by addition of KOH. ,
Kanamycin (2R,3S,4S,5R,6R)-2-(aminomethyl)-6-[(1R,2R,3S,4R,6S)-4,6-diamino-3-[(2S,3R, 4S,5S,6R)-4-amino-3,5-dihydroxy-6-(hydroxymethyl)oxan-2-yl]oxy-2-hydroxycyc lohexyl]oxyoxane-3,4,5-triol ,
Lysozyme Lysozyme Preparation 5 mg/mL Lysozyme soln. * Solve 50 mg of lysozyme in * Add upto 10 mL. * Dispense into 1 mL tubes. * Store at -20 °C :!: Repetitive melting and freezing degrades the activity of lysozyme. References * Lysozyme - , ,
PCI extraction PCI extraction PCI (phenol-chloroform-isoamylalchol) extraction is applied to remove protein portion in the solution to extract nucleicacids. Procedure * Add PCI solution to DNA solution. * Close lid tightly and vortex briefly. * Centrifuge for 5-15min at 10,000 rpm.
Penicillin Penicillin Penicillin G A penicillin derivative commonly used in the form of its sodium or potassium salts in the treatment of a variety of infections. It is effective against most gram-positive bacteria and against gram-negative cocci. It has also been used as an experimental convulsant because of its actions on GAMMA-AMINOBUTYRIC ACID mediated synaptic transmission. ,
Phosphate buffer Phosphate buffer Phosphate buffer is often used for column chlomatography. But it is not an ideal buffer for biological assays because phosphate can act as either a substrate or a regulation factor of enzymes. Besides, the buffering ability decreases significantly at low pH. ,
Sodium acetate Sodium acetate The trihydrate sodium salt of acetic acid, which is used as a source of sodium ions in solutions for dialysis and as a systemic and urinary alkalizer, diuretic, and expectorant. Preparation 3M NaOAc * Solve Sodium acetate trihydrate 204.1g (1.5 mol) in ,
Sodium acetate trihydrate Sodium acetate trihydrate 3M NaOAc preparation * Solve 204.1g (1.5 mol) of Sodium acetate trihydrate in approx. 400mL of * Adjust pH by Acetic acid (pH 5.2) * Add upto 500mL * Sterilization, store at 4 ºC Links * Acetic acid * Sodium acetate * Ethanol precipitation preparation
Streptomycin Streptomycin An antibiotic produced by the soil actinomycete Streptomyces griseus. It acts by inhibiting the initiation and elongation processes during protein synthesis. (MeSH) Preparation * Solve Streptomycin sulfate 500mg in or 20% EtOH aq. * Adjust the volume to 50mL. * Sterilize using filter and dispense into 1mL vials. ,
TAE buffer TAE buffer TAE buffer is Tris-acetate buffer containing EDTA. It is used for agarose gel electrophoreses. Preparation 50x TAE Mix the following solutes and adjust to 1L by . Store this stock solution at room temperature and dillute on your using. ,
TBE buffer TBE buffer TBE buffer is Tris-borate buffer containing EDTA for gel electrophoreses. Preparation 10x TBE Mix the followings and adjust the volume to 1L. Store at room temperature and dillute on your using. Tris108 gBoric acid55 gEDTA・2Na (2)3.7 gConcentration ,
TE buffer TE buffer This solution is used for stock of DNA. EDTA is strong chelator of metal ions that are cofactors of endonucleases. Preparation * Mix two stock solution, 1M Tris-HCl and 0.5M EDTA. * Add upto 1L. stock solution 1M Tris-HCl (pH8.0)10 mL0.5 M EDTA (pH8.0)2 mL => final concentration: 10 mM Tris-HCl, 1 mM EDTA pH 8.0 ,
Tween 20 Tween 20 Sorbitan mono-9-octadecanoate poly(oxy-1,2-ethanediyl) derivatives; complex mixtures of polyoxyethylene ethers used as emulsifiers or dispersing agents in pharmaceuticals. preparation detergent ,