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Restriction enzyme

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AatII AatII Recognition [GACGTC] Temp. 37 ºC BufferLowMidHighAcKCl ≥75% X X X O X Links * Restriction enzyme * Buffer for restriction enzymes * REBASE restriction_enzyme 3_sticky ,
AccI AccI Recognition [GTMKAC] Temp. 37 ºC BufferLowMidHighAcKCl ≥75% X X X O X * GTATAC / GTCGAC * M,K Links * Restriction enzyme * HincII * SalI * Buffer for restriction enzymes * REBASE restriction_enzyme 5_sticky ,
AluI AluI Recognition [AGCT] Temp. 37 ºC BufferLowMidHighAcKCl ≥75% X O X O X Links * Restriction enzyme * Buffer for restriction enzymes * REBASE restriction_enzyme blunt ,
ApaI ApaI Recognition [GGGCCC] Temp. 25 ºC BufferLowMidHighAcKCl ≥75% O X X O X Links * Restriction enzyme * Buffer for restriction enzymes * REBASE restriction_enzyme 3_sticky not_37_c_reaction , ,
AvaI AvaI Recognition [CYCGRG] Temp. 37 ºC BufferLowMidHighAcKCl ≥75% X X X O X * GTCGAG / CCCGGG * R,Y Links * Restriction enzyme * SmaI * Buffer for restriction enzymes * REBASE restriction_enzyme 5_sticky ,
AvaII AvaII Recognition [GGWCC] Temp. 37 ºC BufferLowMidHighAcKCl ≥75% X O X O X * GGACC / GGTCC * W Links * Restriction enzyme * Buffer for restriction enzymes * REBASE restriction_enzyme 5_sticky ,
BamHI BamHI Recognition [GGATCC] Temp. 37 ºC BufferLowMidHighAcKCl ≥75% X O X X X Links * Restriction enzyme * M.BamHI * Buffer for restriction enzymes * REBASE restriction_enzyme 5_sticky ,
BsmI BsmI Recognition [GAATGCN] Temp. 65 ºC BufferLowMidHighAcKCl ≥75% O O O X X * Origin:Bacillus stearothermophilus NUB36 Links * Restriction enzyme * Buffer for restriction enzymes * REBASE restriction_enzyme 3_sticky not_37_c_reaction , ,
BstEII BstEII Recognition [GTNACC] Temp. 60 ºC BufferLowMidHighAcKCl ≥75% X X X O X * Nucleotide N * Origin:Bacillus stearothermophilus ET Links * Restriction enzyme * Buffer for restriction enzymes * REBASE restriction_enzyme 5_sticky not_37_c_reaction , ,
DpnI DpnI DpnI cuts sites 'GATC' if the adenine is methylated. Recognition [GATC] Temp. 37 ºC BufferLowMidHighAcKCl ≥75% X X X O X Reaction DpnI cuts sites 'GmATC' (mA: N6-methyladenine). The structures below are part of dam-methylated pUC19 and non-methylated pUC19, respectively. ,
DraI DraI Recognition [TTTAAA] Temp. 37 ºC BufferLowMidHighAcKCl ≥75% X O X O X Links * Restriction enzyme * Buffer for restriction enzymes * REBASE restriction_enzyme blunt ,
HaeII HaeII Recognition [RGCGCY] Temp. 37 ºC BufferLowMidHighAcKCl ≥75% X O X O X * GGCGCC/AGCGCT * Nucleotide M,K Links * Restriction enzyme * Buffer for restriction enzymes * REBASE restriction_enzyme 3_sticky ,
HhaI HhaI Recognition [GCGC] Temp. 37 °C BufferLowMidHighAcKCl ≥75% X O O O X Links * Restriction enzyme * Buffer for restriction enzymes * REBASE restriction_enzyme 3_sticky ,
HincII HincII Recognition [GTCGAC] Temp. 37 °C BufferLowMidHighAcKCl ≥75% reactive X O O O X Links * Restriction enzyme * SalI * AccI * Buffer for restriction enzymes * REBASE restriction_enzyme blunt ,
HindIII HindIII Recognition [AAGCTT] Temp. 37 °C BufferLowMidHighAcKCl ≥75% X O X X X Links * Restriction enzyme * Buffer for restriction enzymes * REBASE restriction_enzyme 5_sticky ,
KpnI KpnI Recognition [GGTACC] Temp. 37 °C BufferLowMidHighAcKCl ≥75% O X X O X Links * Restriction enzyme * Buffer for restriction enzymes * REBASE restriction_enzyme 3_sticky ,
MboI MboI Recognition [GATC] Temp. 37 ºC BufferLowMidHighAcKCl ≥75% X O O O O Isoscizomer Sau3AI Sau3AI is an isoscizomer of MboI. Sau3AI can cut methylated sites, so it is often used for partial digestion of genome DNA. Links * Restriction enzyme * Sau3AI * DpnI * ,
MspA1I MspA1I Recognition [CMGCKG] Temp. 37 °C BufferLowMidHighAcKCl ≥75% X X X O X * CAGCTG / CCGCGG * Nucleotide M,K Links * Restriction enzyme * Buffer for restriction enzymes * REBASE restriction_enzyme blunt ,
NarI NarI Recognition [GGCGCC] Temp 37 °C BufferLowMidHighAcKCl ≥75% O X X X X Links * Restriction enzyme * Buffer for restriction enzymes * REBASE restriction_enzyme 5_sticky ,
NdeI NdeI Recognition [CATATG] Temp. 37 °C BufferLowMidHighAcKCl ≥75% X X O O X Links * Restriction enzyme * Buffer for restriction enzymes * REBASE restriction_enzyme 5_sticky ,
NsiI NsiI Recognition [ATGCAT] Temp. 37 ºC BufferLowMidHighAcKCl ≥75% X X O X X Links * Restriction enzyme * Buffer for restriction enzymes * REBASE restriction_enzyme 3_sticky ,
PstI PstI Recognition [CTGCAG] Temp. 37 °C BufferLowMidHighAcKCl ≥75% X X O X X Links * Restriction enzyme * Buffer for restriction enzymes * REBASE restriction_enzyme 3_sticky ,
PvuI PvuI Recognition [CGATCG] Temp. 37 °C BufferLowMidHighAcKCl ≥75% X X O X O with BSA Links * Restriction enzyme * Buffer for restriction enzymes * REBASE restriction_enzyme 3_sticky ,
PvuII PvuII Recognition [CAGCTG] Temp. 37 °C BufferLowMidHighAcKCl ≥75% O O O X O Links * Restriction enzyme * AluI * MspA1I * Buffer for restriction enzymes * REBASE restriction_enzyme blunt ,
RsaI RsaI Recognition [GTAC] Temp. 37 °C BufferLowMidHighAcKCl ≥75% O O X O X Links * Restriction enzyme * Buffer for restriction enzymes * REBASE restriction_enzyme blunt ,
SacI SacI Recognition [GAGCTC] Temp. 37 °C BufferLowMidHighAcKCl ≥75% O X X O X Links * Restriction enzyme * Buffer for restriction enzymes * REBASE restriction_enzyme 3_sticky ,
SalI SalI Recognition [GTCGAC] Temp. 37 °C BufferLowMidHighAcKCl ≥75% reactive X X O X X Links * Restriction enzyme * HincII * AccI * TaqI * Buffer for restriction enzymes * REBASE restriction_enzyme 5_sticky ,
Sau3AI Sau3AI Recognition [GATC] Temp. 37 ºC BufferLowMidHighAcKCl ≥75% O O O O X Isoscizomer The recognition sites of isoscizomer, MboI will be blocked by dam methylase. On the other hand, Sau3A can cut the GATC sites even if the adenines are methylated. Therefore, Sau3AI is used for mapping or construction of library of methylated genome. ,
ScaI ScaI Recognition [AGTACT] Temp. 37 °C BufferLowMidHighAcKCl ≥75% o o O X X Links * Restriction enzyme * Buffer for restriction enzymes * REBASE restriction_enzyme blunt ,
SmaI SmaI Recognition [CCCGGG] Temp. 25 ºC BufferLowMidHighAcKCl ≥75% X O O O X Links * Restriction enzyme * AvaI * Buffer for restriction enzymes * REBASE restriction_enzyme blunt not_37_c_reaction , ,
SphI SphI Recognition [GCATGC] Temp. 37 °C BufferLowMidHighAcKCl ≥75% O O X O X Links * Restriction enzyme * Buffer for restriction enzymes * REBASE restriction_enzyme 3_sticky ,
SspI SspI Recognition [AATATT] Temp. 37 °C BufferLowMidHighAcKCl ≥75% X o X X X Links * Restriction enzyme * Buffer for restriction enzymes * REBASE restriction_enzyme blunt ,
Star activity Star activity It has been demonstrated that under extreme non-standard conditions, restriction endonucleases are capable of cleaving sequences which are similar but not identical to their defined recognition sequence. This altered or relaxed specificity has been termed “star” activity.
StuI StuI Recognition [AGGCCT] Temp. 37 °C BufferLowMidHighAcKCl ≥75% o O X o X Links * Buffer for restriction enzymes * REBASE restriction_enzyme blunt ,
TaqI TaqI Recognition [TCGA] Temperature 65 ºC BufferLowMidHighAcKCl ≥75% X X O O X * Origin: Thermus aquaticus YTI Links * Buffer for restriction enzymes * REBASE restriction_enzyme 5_sticky not_37_c_reaction , ,
XbaI XbaI Recognition [TCTAGA] Temp. 37 °C BufferLowMidHighAcKCl ≥75% X O X X X Links * Restriction enzyme * Buffer for restriction enzymes * REBASE restriction_enzyme 5_sticky ,